Fig 4. Tyrosine residues Y1096 and Y1282, and to a lesser extent Y1092 are phosphorylated on the pA fragment during the kinase reaction.

A red circle indicates a phosphorylated tyrosine residue. Green indicates the Flag tag residues. Red indicates HIS6 tag and tyrosine residues (Y). Blue indicates the ALK fragment residues. Three μg of protein was run on the 1D gels used for pA band cutting.