Fig 5. Representative images of 1D pTyr WBs run on separate days in triplicate (Runs 1 and 2).

Both sets were loaded with increasing amounts of pA (Lot 1 for Run 1 and Lot 2 for Run 2) as follows: lane 3–4, 0.1 ng; lanes 5–6, 0.3 ng; 7–8, 0.5 ng; 9–10, 1.0 ng; 11–12, 2.0 ng, and 13–14, 4.0 ng. Run 1 gels were loaded with 5 μl pE Lot 1 in even lanes; Run 2 gels were loaded with 5 μl pE Lot 2 in even lanes. Proteins in the 1D gels were transblotted onto PVDF followed by pTyr WB as described in methods. Three and 10-min films were obtained for each WB; 10-min films from the second gel for each run are shown.