Figure 6.
Reversibility of the vesicle coating process. (a) Schematic representation of the VLT1s’ disassembly through a toehold-mediated displacement reaction. A 10 nt toehold was included in the T1 triskelion (referred to as T1s) hybridizing with a displacement strand S. (b) Time-resolved and relative fluorescence quenching after addition of S to VLT1s in comparison to a nonhybridizing Scontrol (Sc-), and the effect presented by dilution with PBS (the data represent averages of three sample preparations; error bars show the standard deviation). (c) Cryo-EM images of VLT1s before (left) and after addition of S (right). Scale bars: 50 nm. (d) Top panels: size (hydrodynamic diameters by DLS from counts in intensity) of VLT1s before and after the addition of S, and the subsequent addition of the original T1. The bottom panels collect the DLS data of each sample after the addition of 0.5% TX100.