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. 2018 Oct 9;100(2):390–397. doi: 10.1093/biolre/ioy221

Figure 2.

Figure 2.

Cell fusion in endometriosis-like lesions in the whole body model. (A–C) Flow cytometric analysis of GFP+ cells (A) in the uterus of a female offspring resulting from the cross β-actin-Cre X ZsGreen1 (positive control), (B) in ZsGreen1 lesions implanted within a wild-type mouse (negative control) at 4 weeks post EI, and in (C) ZsGreen1 lesions implanted within β-actin-Cre mouse at 4 weeks post EI. n = 10 mice in each group. (D) Representative image of GFP immunostaining in control endometriosis lesions at 4 weeks post EI. (E) Representative images of GFP immunostaining in experimental lesions showing GFP+ cells (brown) at 4 weeks post EI. Bottom panel: higher magnification images of dashed areas. Red arrows point to fusion-derived cells. n = 6–12 mice in each group. *P < 0.02. Scale bar = 50 μm. (F) Quantification of GFP+ cells in mouse lesions in immunohistochemical sections from the whole body model (left column) and BMT model (right column). n = 5 mice in each group.