Table 2. Summary of serological and molecular diagnostic results for the 5 patients with neurological manifestations.
| Patient ID | Sample source | No. of days of illness | NS1 Antigen rapid test | Viremia level by real time RT-PCR (log10copies/ml) | Virus isolation | DENV conventional RT-PCR | NGS analysis | IgM ratioa | IgG titerb | Type of infection | Remark |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 252-Sc | 3 | + | 5.7 | + | DENV-2 | DENV-2 | 11.6 | 43,002 | Sec | recovery |
| 252-Cd | 5 | ND | 7.8 | + | DENV-2 | DENV-2 | 3.2 | 3,666 | |||
| 2 | 257-S | 5 | + | 8.9 | + | DENV-2 | DENV-2 | 0.5 | 294 | Pri | death |
| 257-C | 5 | + | 8.3 | + | DENV-2 | DENV-2 | 0.5 | 95 | |||
| Brain tissue (autopsy) | 7 | DENV-2 | |||||||||
| 3 | 256-S | 5 | + | 6.7 | + | DENV-2 | DENV-2 | 9.5 | 44,876 | Sec | recovery |
| 256-C | 5 | ND | 3.4 | - | - | - | 12.1 | 57,677 | |||
| 4 | 251-S | 3 | + | 4.2 | - | - | - | 11.5 | 72,099 | Sec | recovery |
| 251-C | 3 | ND | 6.3 | - | - | - | 4.5 | 8,760 | |||
| 5 | 1354-S | 10 | - | 3.6 | - | - | - | 11.8 | 60,405 | Sec | recovery |
| 1354-C | 10 | - | 3.9 | - | - | - | 2.2 | 16,183 |
ND: not done. Pri: primary infection. Sec: secondary infection.
a DENV IgM P/N ratio ≥ 2 considered as positive.
b DENV IgG titer ≥ 29,000 considered as secondary and < 29,000 as primary infection.
cS: serum
dC:CSF.