Fig 6. Recruitment of KIF5C to PRV particles requires gE/gI-US9p.

Float-up fractions from P or M PRV-infected differentiated CAD cells were immunostained with a rabbit anti-KIF5C or control IgG (as indicated at top of each panel), imaged in the red (mCherry-tagged capsids) and green (anti-rabbit antibody immunofluorescence) channels and images merged. (A, B) Fields of float-up particles from a P PRV infection stained with control IgG and anti-KIF5C IgG respectively. (C, D) Fields of float-up particles from a M PRV infection stained with control IgG and anti-KIF5C IgG respectively. Four boxed regions (i-iv) in panels B and D were magnified 5-fold and are shown at the bottom of each panel. The individual channel images used to construct the merged panels in A-D are shown in inverted black and white in S4 Fig and S5 Fig. (E) Numbers of P and M red fluorescent particles that exhibit anti-KIF5C staining as a percent of total particles in the field, derived from images similar to those in (B) and (D). Plotted values are mean and standard deviation from the mean for 20,529 P and 16,830 M particles, respectively.