Table 1. Rate of deacylation by MmATD and MmThrRS.
Deacylation rates of L-Ala-tRNAThr (200 nM) by MmATD and MmThrRS. The kobs values are calculated by fitting the deacylation curves (Figures 2A and 3F) to the first-order exponential decay equation using GraphPad Prism.
| Enzymes | 5 mM H2O2 | [Enzyme] | kobs (min−1) |
|---|---|---|---|
| MmATD | - | 1 nM | 0.127 ± 0.004 |
| MmATD | + | 1 nM | 0.152 ± 0.005 |
| MmThrRS | - | 10 nM | 0.142 ± 0.004 |
| MmThrRS | + | 10 nM | No activity |