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. 2020 May 28;9:e58118. doi: 10.7554/eLife.58118

Table 1. Rate of deacylation by MmATD and MmThrRS.

Deacylation rates of L-Ala-tRNAThr (200 nM) by MmATD and MmThrRS. The kobs values are calculated by fitting the deacylation curves (Figures 2A and 3F) to the first-order exponential decay equation using GraphPad Prism.

Enzymes 5 mM H2O2 [Enzyme] kobs (min−1)
MmATD - 1 nM 0.127 ± 0.004
MmATD + 1 nM 0.152 ± 0.005
MmThrRS - 10 nM 0.142 ± 0.004
MmThrRS + 10 nM No activity