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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: FASEB J. 2020 May 4;34(6):8296–8309. doi: 10.1096/fj.202000214RR

FIGURE 7.

FIGURE 7

Overexpression UT stimulated fibroblast activation to myofibroblasts in primary cultured cardiac fibroblasts. (A, B & C) cultured fibroblasts treated with adenovirus to overexpress UT. Quantitative mRNA expression in these cells performed by real-time PCR. All gene expressions were calculated by ΔΔcq and standardized by housekeeping gene 18s. A, Renin; B, Ace (angiotensin-converting enzyme); and C, Agtr1: angiotensin II receptor type1. D, Primary cultured cardiac fibroblasts transduced with Ad-UT for 48 hours. Western blot of lysates were performed for α-SMA and TGF. The point graphs show the levels of each protein comparing in arbitrary units. Protein level normalized by total protein density of Ponceau S staining. All data: mean ± SEM (N = 4-6). *P < .05, **P < .005 by one-way ANOVA analysis