. Author manuscript; available in PMC: 2021 Jun 1.

Published in final edited form as: FASEB J. 2020 May 4;34(6):8296–8309. doi: 10.1096/fj.202000214RR

TABLE 1.

Phenotypic characteristics of WT and 5/6Nx mice

Parameter	Sham	Sham + DMTU	5/6Nx + vehicle	5/6Nx + DMTU
Initial BW (g)	25.8 ± 0.4	25.0 ± 0.6	26.5 ± 0.6	26.0 ± 0.5
Final BW (g)	29.8 ± 0.4	25.9 ± 1.1^*	27.6 ± 0.7^*	26.4 ± 0.5^*
BUN (mg/dL)	24.9 ± 1.5	23.3 ± 2.1	50.1 ± 1.6^*^†	47.2 ± 3.3^*^†
Urine urea (mmol/L)	1510 ± 154	1555 ± 128	761 ± 48^*^†	926 ± 95^*^†
24 hr intake volume/BW (mL/g)	0.159 ± 0.028	0.129 ± 0.006	0.324 ± 0.012^*^†	0.265 ± 0.028^*^†
24 hr Urine volume/BW (mL/g)	0.023 ± 0.004	0.058 ± 0.010^*	0.148 ± 0.008^*^†	0.170 ± 0.013^*^†#
Water in-out balance/BW (mL/g)	0.145 ± 0.010	0.081 ± 0.010^*	0.185 ± 0.013^†	0.114 ± 0.014^‡
24 hr Urine osmolality (mOsmol/kg H₂O)	3595 ± 559	2378 ± 133^*	908 ± 40^*^†	1206 ± 116^*^†#
Plasma osmolality (mOsmol/kg H₂O)	322 ± 2	322 ± 1	330 ± 2	329 ± 3

Note: Volume in-out balance/BW was calculated by [(24 hr intake volume) − (24hr urine volume)]/BW, All data; mean ± SEM (N = 5-8).

Abbreviations: BW, body weight, BUN, blood urea nitrogen.

P < .05 vs sham + vehicle;

^†

P < .05 vs sham + DMTU;

^‡

P < .05 vs 5/6Nx + vehicle by one-way ANOVA with Bonferroni test;

P < .05 for interaction between 5/6Nx and DMTU (P < .0001 in 24 hr urine volume/BW and 24 hr urine osmolality for the factor of 5/6Nx; P < .05 in 24 hr urine volume/BW and 24 hr urine osmolality for the factor of DMTU) by two-way ANOVA.