(a) Voxel position in the anterior cingulater cortex and medial prefrontal cortex overlaid on a sagittal, coronal, and axial T1-weighted image. The voxel-of-interest was aligned with the interhemispheric fissure in the transverse and coronal planes, while placed anterior to the genu of the corpus callosum in the sagittal plane to mainly include the anterior cingulate cortex. (b) Edited spectrum and model fit of the Glx peak at 3.75ppm and GABA peak at 3ppm using Gannet 3.0. Images were obtained from a representative study participant. GABA, gamma-aminobutyric acid; Glx, glutamate+glutamine