Figure 2:

Average total activity for every 6 minutes for the day with saline injection for BXD40f on Iron-sufficient (control, n=6) and iron-deficient (ID, n=7) diets.

Lights off active period is from 5 AM to 5 PM shown by the dark bar at the top of the figure. Note the control replicates the increased activity to the levels seen earlier in the day for the 2-hour period before lights on (17:00 – 05:00) reported in a prior study (Earley et al, 2019) and shown in figure 1. The ID show the same pattern except it starts about 2 hours earlier and persists even into the very first part of the inactive period. Overall activity during the active period is increased for the ID compared to the control but this is a consistent statistically significant difference only during 13:06 – 14:36, the 4^th and 3^rd hours before end of the active cycle.