Fig. 2.

Phosphorylation of BTK in B cell subsets. a Unstimulated CD73^neg B_eff cells showed a significantly higher phosphorylation of BTK than CD73⁺ B_reg two hours after isolation and staining. After pre-treatment with ADO for 2 h, BTK phosphorylation was reduced in CD73neg B_eff cells. Data shown as median with interquartile range; paired t-test; n = 5. b Isolated human B cells were first stained for CD73 and then incubated with ADO for two hours. Phosphorylation was measured after stimulation with anti-µ-F(ab’)₂. Incubation with ADO reduced BTK phosphorylation in CD73^neg B_eff cells. Analyzed with a two-way ANOVA; n = 5. c Expression of IgM and CD73 on CD19⁺ B cells in the density plot of one representative patient. d Frequency of IgM⁺ cells in the CD73^neg B_eff and CD73⁺ B_reg subset