Fig. 2. 4MSOB-ITC is degraded by the fungus S. sclerotiorum.

a Quantification of 4MSOB-ITC in A. thaliana plants with and without S. sclerotiorum. Data represent mean ± SEM (n = 4 inoculated plants) and were analyzed by a Kruskal–Wallis rank sum test (p < 0.01) followed by a Games–Howell post-hoc test. Different letters above the bars indicate significant differences at p < 0.05. b Quantification of 4MSOB-ITC in fungal cultures during a time course. Twenty-five micromolar 4MSOB-ITC was used for each fungal culture. Data were analyzed by a linear regression (R² = 0.94, p < 0.001). Quantification of c 4MSOB-ITC mercapturic acid pathway conjugates, and d 4MSOB-ITC hydrolytic degradation products 4MSOB-amine and 4MSOB-acetamide, in the fungus-inoculated liquid medium supplemented with 4MSOB-ITC. Data represent mean ± SEM (n = 3 independent fungal cultures) and were analyzed by one-way ANOVA (p < 0.001) followed by Tukey’s post-hoc test. Different letters above the bars indicate significant differences at p < 0.05. e Proposed pathways for the degradation of 4MSOB-ITC by S. sclerotiorum. 4MSOB-ITC, 4-methylsulfinylbutyl isothiocyanate; 4MSOB-GSH, 4MSOB-ITC glutathione conjugate; 4MSOB-CG, 4MSOB-ITC cysteinylglycine conjugate; 4MSOB-CYS, 4MSOB-ITC cysteine conjugate; 4MSOB-NAC, 4MSOB-ITC N-acetylcysteine conjugate; 4MSOB-amine, 4-methylsulfinylbutylamine; 4MSOB-acetamide, 4-methylsulfinylbutylacetamide. Source data are provided as a Source data file.