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. 2020 Jun 18;11:3090. doi: 10.1038/s41467-020-16921-2

Fig. 3. Ss12040 is a SaxA candidate gene in S. sclerotiorum.

Fig. 3

a Maximum likelihood tree of fungal metallo-β-lactamase-like enzymes with similarity to known bacterial ITCases. Amino acid sequences were aligned with MEGA, and phylogenetic trees were constructed with PhyML using 1000 bootstraps. Numbers on each branch in the ML tree signify bootstrap values. b Induction of SaxA candidate gene Ss12040 expression by 4MSOB-ITC in vitro. The fungal culture medium was supplemented with 4MSOB-ITC (25 µM), with only ethanol being added to the control medium. Gene expression was determined 30 min post inoculation. Data represent mean ± SEM (n = 3 independent fungal cultures; in vitro induction of Ss12040 by 4MSOB-ITC was repeated once) and were analyzed by a two-tailed Student’s t test (**p < 0.01). c Expression of SaxA candidate gene Ss12040 24 hpi of different A. thaliana lines. Data represent mean ± SEM (n = 6 independent inoculated plants) and were analyzed by a Kruskal–Wallis rank sum test (p < 0.001) followed by a Games–Howell post-hoc test. Different letters above the bars indicate significant differences at p < 0.05. 4MSOB-ITC, 4-methylsulfinylbutyl isothiocyanate. Source data are provided as a Source data file.