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. 2020 Jun 18;10:9940. doi: 10.1038/s41598-020-66852-7

Figure 5.

Figure 5

Thermal stability of solubilized MsbA. Purified MsbA was studied in detergent (Det) and zSMALPs (solubilized from proteoliposomes). For these experiments the samples were heated to 65 °C for 15 min and MsbA in the supernatant, after centrifugation at 100,000 g for 45 min, was quantified on Western blots probed with an anti-His antibody. The 1:1 and 2:1 zSMALPs were prepared using the corresponding zSMAs derived from RAFT P(St-ran-MA) copolymers with molecular weights of 6.7 and 6.4 kDa, respectively. *Denotes P < 0.001 vs both copolymers; †Denotes P < 0.002 vs 2:1 zSMA. Data are means ± SEM (n = 4 per condition).