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. 2020 Jun 18;10:9898. doi: 10.1038/s41598-020-66728-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Global analyses of gene expression. (a) Top 250 most differentially expressed genes among 8 cell subtypes isolated from blood and brain. (b) PCA plot illustrates difference in principle components between brain and blood samples. (c) MA plot reveals the number of genes with a significant change in expression in comparison between GFP^pos and GFP^neg brain monocytes. (d) Significantly differentiated gene expression shown in MA plot between blood-derived classical infiltrating monocytes (Ly6C^high) and patrolling monocytes (Ly6C^low). (e) Significantly differentiated gene expression shown in MA plot between blood-derived monocytes and glioma monocytes.