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. 2020 Jun 18;10:9919. doi: 10.1038/s41598-020-66916-8

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-dependent cation concentration (C) profiles measured from the S2 cell wall layer in (a) KCl 75% RH and (b) CuCl₂ 75% RH specimens as a function of distance (x) and time (t). In the KCl 70% RH specimen (a), the average K C remained unchanged up to distance x = 0, and then increased with increasing t for x > 0. This behavior is indicative of infinite source diffusion. In the CuCl₂ 75% RH specimen (b), with increasing t the Cu C decreased for x < 0 and increased for x > 0. This behavior is indicative of finite source diffusion. For both series the C were measured at times 0, 23, 45, 68, 90, 112, 135, and 159 min. Each colored line represents one time, and the arrows point to increasing time. The indicated parameters C_o and C_s were used in the diffusion constant analysis developed in the Supplementary Information and are described in the text.