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. 2020 Jun 18;11(6):469. doi: 10.1038/s41419-020-2663-1

Fig. 2. Effect of NaB on high-cholesterol-induced BACE1 expression and Aβ accumulation.

Fig. 2

a SK-N-MC cells were treated with high cholesterol (25 µM) for various time (0–48 h). APP and BACE1 were analyzed by western blot. β-actin was used as a loading control. n = 4. b Cells were pretreated with NaB (500 µM) for 30 min prior to treatment of high cholesterol for 24 h. The mRNA expression levels of APP, BACE1, and PSEN1 were analyzed by quantitative real-time PCR. Data were normalized by the ACTB mRNA expression levels. n = 4. c Cells were pretreated with NaB for 30 min prior to treatment of high cholesterol for 24 h. The expression levels of APP, BACE1, and PSEN1 were analyzed by western blot. β-actin was used as a loading control. n = 4. d Cells were pretreated with NaB for 30 min prior to treatment of high cholesterol for 24 h and immunostained with BACE1 antibody. Scale bars are 8 µm (magnification, ×1,000). n = 3. e Cells were pretreated with NaB, NaP (500 µM), and NaA (500 µM) for 30 min prior to treatment of high cholesterol for 24 h. The expression levels of APP, BACE1, and PSEN1 were analyzed by western blot. β-actin was used as a loading control. n = 3. f Cells were pretreated with NaB for 30 min prior to treatment for high cholesterol for 72 h. Aβ concentration of medium samples was detected by using ELISA kit. Data are presented as a mean ± S.E.M. n = 4. *p < 0.05 versus control, #p < 0.05 versus high-cholesterol treatment. All blot and immunofluorescence images shown are representative.