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. 2020 Jun 18;11:3086. doi: 10.1038/s41467-020-16896-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — X-axis shows the foliar concentration of the AmGSTF1 protein, while Y-axis represents herbicide resistance as the proportional (%) survival of individuals for (a) the ‘SU’ herbicide mesosulfuron, (b) the ‘Fop’ fenoxaprop and (c) the ‘Dim’ cycloxydim. Fitted lines show the mean predicted relationship following mixed model analysis given in Supplementary Table 2, with shaded regions representing the estimated 95% confidence interval of the prediction. The modelled response variable was the proportional survival of individuals (n = 18) over all doses of the tested herbicide. Terms for the target-site resistance frequency, AmGSTF1 concentration and their interaction were included as fixed factors, with each model containing random effect terms for the population ID and herbicide dose. Solid black lines are used for significant relationships in (a) (p ≤ 0.001) and (b) (p ≤ 0.001), while a dashed line is used where the relationship was non-significant in (c) (p = 0.10 ns).