Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 18;10:9921. doi: 10.1038/s41598-020-64257-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The differential expression of key early and late ovulatory genes in ovaries of gcCbfb;Runx2KO mice. (A) Ovaries were collected at 3 h after hCG administration. The levels of mRNA for early ovulatory genes were measured by qPCR, normalizing to the Rpl19 value in each sample (n = 7 and 6 for control and mutant mice, respectively). *p < 0.05. (B) Ovaries were collected at 11 h after hCG administration. The levels of mRNA for late ovulatory genes were measured by qPCR, normalizing to the Rpl19 value in each sample (n = 8 and 7 for control and mutant mice, respectively). **p < 0.01. (C & D) The localization of Pgr mRNA and Ptgs2 mRNA was evaluated via in situ hybridization analyses in ovaries collected at 3 and 11 h post-hCG (n = 3/genotype). Green fluorescence staining represents transcripts for these genes. The tissue sections were counterstained with propidium iodide (red). Arrowheads and arrows point to granulosa cells of preovulatory follicles and expanded cumulus cells, respectively. Scale bars, 500 μm for all the images.