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. 2020 Jun 18;10:9921. doi: 10.1038/s41598-020-64257-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of vascularization and apoptosis during the post-ovulatory period in gcCbfb;Runx2KO mice. Ovaries were collected at 24, 48, and 72 h after hCG administration (n = 3 animals/ genotype). (A) Immunohistochemical analysis was used to detect PECAM-1 (green fluorescence staining). The ovarian sections were counterstained with propidium iodide (red). (B) TUNEL assay was used to detect apoptotic cells (brown staining). The ovarian sections were counterstained with hematoxylin (blue). Arrows point to CLs in control animals and the symbol (*) was placed in unruptured follicles and poorly luteinized collapsed follicles in gcCbfb;Runx2KO mice. Scale bars, 250 μm for all the images.