Fig. 3. The MultiPower approach.

a Relationship between omic platforms (MS in blue and sequencing in red), FoM (purple), power parameters (green), and MultiPower. MS- and seq-based omics have different key properties that determine their FoM, which in turn affect differently to power parameters used as input by MultiPower to compute sample size in multi-omics experiments. b Overview of the MultiPower method. MultiPower works either with no prior data and with pilot data (red boxes). The blue box contains the parameters to be set by users. Green, orange, and purple boxes represent the data types accepted by MultiPower and the rest of power parameters estimated by MultiPower from prior data when available or given by the user. MultiPower solves an optimization problem to estimate the optimal sample size and to provide a power study of the experiment (gray boxes).