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. 2020 Jun 18;11:3095. doi: 10.1038/s41467-020-16807-3

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Structure of the pBXL{attP_PUbDsRed.T3_fa_s1-tTA_a} tTA driver and pBac{3xP3-ECFPaf; 5’HS4>5’HS4>TREp-hid^Ala5>5’HS4>5’HS4} lethal effector vectors in the double-homozygous line, DH-1. b Images of an adult DH-1 male and w[m] female under brightfield (BF) and Texas Red (DsRed) and CFP (ECFP) epifluorescence optics. DH-1 males, having a w[m] background, exhibit the white^- eye phenotype under brightfield and whole-body PUbDsRed and eye-specific 3xP3-ECFP fluorescent markers for the driver and effector vectors, respectively. Non-transgenic w[m] females exhibit the white^- eye phenotype under brightfield and are non-fluorescent for both markers.