Table 1.

Tet-off lethality revertant adult survivors on Tet-free diet.

Matings	No. of P1 matingsa	Diet	F1 R/C survivors	Fertile F1 R/C survivors	F2 R/C survivor linesb	Primary-site revertants	Second-site revertants
Control	6	Tet	90,006c	—	—	—	—
Experimental	80	Tet-free	109	73	20	7	13
Survival frequencyd			9.1 ± 0.45 × 10−5	6.1 ± 0.30 × 10−5	1.7 ± 0.083 × 10−5	5.8 ± 0.29 × 10−6	1.1 ± 0.054 × 10−5
Modified survival frequencye			8.9 ± 0.44 × 10−5	5.9 ± 0.29 × 10−5	1.4 ± 0.071 × 10−5	3.3 ± 0.17 × 10−6	1.1 ± 0.054 × 10−5

^a400 w[m] females mated to 100 DH-1 double homozygous driver/effector (PUb-DsRed/3xP3-Cyan; R/C) males.

^bF₂ R/C progeny from individual fertile F₁ R/C backcross matings to w[m].

^cCalculated from 15,001 ± 389 SEM surviving F₁ adults per control mating.

^dNo. of R/C marked F₁ survivors/~1,200,000 ±SEM adults screened (estimation based on surviving F₁ adults from each control mating on Tet-diet).

^eModified survival frequency data based on the deletion of the primary-site revertant lines 9-f1, 62-f1, and 65-m2 whose survival may have resulted from cis-recombination between the 5’ HS4 repetitive insulator sequences that are not required for lethality system function, which does not affect the second-site revertant frequency; frequencies presented as confidence intervals at the 95% confidence level.