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. 2020 Jun 18;11:3088. doi: 10.1038/s41467-020-16903-4

Fig. 3. Effect of ribonucleotides on resection by BLM-DNA2.

Fig. 3

a BLM and DNA2 (10, 20, 30, 40 nM) along with RPA (12.5 nM) were incubated with the indicated substrate (2.5 nM) at 37 °C for 10 min. Electrophoresis and quantification were performed as in Fig. 1a. b DNA unwinding by BLM (20, 40, 60 nM) was monitored in the presence of RPA (12.5 nM). Reaction mixtures were resolved in a 10% native acrylamide gel. In the lane labeled HD, the substrate was heat denatured by incubation at 95 °C for 2 min. The dsDNA and ssDNA bands were quantified and data were graphed. c Substrate unwinding by BLM (25, 50 nM) in the presence of RPA (12.5 nM) was examined as in (b). d Substrate unwinding by BLM (5, 10, 20 nM) was examined in the presence of RPA (12.5 nM) as in (b). Error bars in all the panels represent the standard deviation of results from n = 3 independent experiments, and all points represent the mean. RNA is denoted in red, and the asterisk indicates the location of the radiolabel.