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. 2020 Jun 18;11:3088. doi: 10.1038/s41467-020-16903-4

Fig. 4. DNA2 cannot cleave within stretches of RNA.

Fig. 4

a DNA2 (0.25, 0.5, 0.75 nM) was incubated with the indicated Y-shaped substrate (2.5 nM) at 37 °C for 5 min in the presence or absence of 2 mM ATP. Reaction mixtures were resolved in a 20% denaturing polyacrylamide gel. b The same assay was done in the presence of RPA (10 nM). Reaction mixtures were resolved in a 20% denaturing polyacrylamide gel. Error bars in all the panels represent the standard deviation of results from n = 3 independent experiments, and all points represent the mean. RNA is denoted in red, and the asterisk indicates the location of the radiolabel.