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. 2020 Jun 18;11(6):473. doi: 10.1038/s41419-020-2674-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a qRT-PCR analysis for the expression level of NORAD in ISK and SPEC-2 EC cell lines with different doses transfection of NORAD, in comparison with the empty vectors. b Cell-counting assays for the control and ectopic NORAD-expressing ISK and SPEC-2 cells after 48 h transfection. c Increased percentage of apoptosis in the ectopic NORAD-expressing EC cells after 48 h transfection via FACS analysis. d TUNEL assays for apoptotic cells in the control and NORAD-expressing EC groups (left). Statistics of the TUNEL-Cy3 positive cells are shown (right). Scale bar, 100 μm. e Activated expression of cleaved PARP and cleaved caspase-3 was visualized by western blot. The results were determined from triplicates, and the error bars represented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001. TUNEL TdT-mediated dUTP Nick-end labeling, GAPDH glyceraldehyde-3-phosphate dehydrogenase.