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. 2020 Jun 18;11(6):473. doi: 10.1038/s41419-020-2674-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a qRT-PCR analysis for the binding of NORAD, SNHG1, and GAPDH expression by an anti-FUBP1 antibody compared with that by an IgG control antibody in 293FT cells. SNHG1 and GAPDH served as the positive and negative controls, respectively. b qRT-PCR analysis for the binding of NORAD on FUBP1 in EC cells with Aza treatment by RIP assays. c Western blot for the FUBP1 protein (lower panel) pulled down by truncated NORAD (NORAD-1, -2, -3, and -4; upper panel). d The expression levels of full-length NORAD and fragments transfected in ISK and SPEC-2 cells were detected by qRT-PCR. e Cell-counting assays for ISK and SPEC-2 cells transfected with full-length NORAD and fragments, respectively. f The percentage of apoptotic cells transfected with full-length NORAD and fragments, evaluated by FACS analysis. g Western blot for the expression of cleaved PARP and cleaved caspase-3 after ectopic expression of full-length NORAD and fragments. The results were determined from triplicates, and the error bars represented as the mean ± SD, */# P < 0.05, **P < 0.01, ***P < 0.001. FUBP1 far upstream element-binding protein 1, SNHG1 small nucleolar RNA host gene 1, RIP RNA immunoprecipitation, YFP yellow fluorescent protein.