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. 2020 Jun 12;11:1115. doi: 10.3389/fmicb.2020.01115

FIGURE 3.

FIGURE 3

The signal peptide (SP) of RsIA_NP8 is functional. (A) Functional validation of the SP of RsIA_NP8 using yeast invertase secretion assay. All transformed YTK12 yeast strains grew on YPRAA media with raffinose as the sole carbon source (1% yeast extract, 2% peptone, 2% raffinose, and 2 μg antimycin A per liter). N-terminal sequences of Phytophthora sojae Avr1b and Magnaporthe oryzae Mg87 were used as positive and negative controls, respectively. The untransformed YTK12 did not grow on either CMD-W (0.67% yeast N base without amino acids, 0.075% tryptophan dropout supplement, 2% sucrose, 0.1% glucose, and 2% agar) or YPRAA media. Yeast growth on CMD-W media was equally viable among the transformed strains. Mg87: negative control Mg87 SPs; Avr1bSP: positive control Avr1b SPs; RsIA_NP8SP: SPs of RsIA_NP8. (B) Functional validation of the SP of RsIA_NP8 by experiments of swap the RsIA_NP8SP with a SP of INF1.