FIGURE 5.

The predicted motifs and signal peptide of RsIA_NP8 are required to induce cell death. (A) Expression of RsIA_NP8 and its mutants in N. benthamiana by agro-infiltration. Typical symptoms were photographed at four dpi. Numbers e.g., 28/30, indicate that 28 of 30 infiltrated leaves exhibiting cell-death or mottling phenotypes. (B) Expression of mutant proteins in infiltrated leaves detected by Western blotting. (C) Functional characterization of three putative N-glycosylation sites for RsIA_NP8. Typical symptoms were photographed at four dpi. Numbers e.g., 28/30, indicate that 28 of 30 infiltrated leaves exhibiting cell-death or mottling phenotypes. (D) Expression of three putative N-glycosylation site mutant proteins in infiltrated leaves detected by Western blotting.