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. 2020 Jun 12;10:287. doi: 10.3389/fcimb.2020.00287

Figure 2.

Figure 2

Glycolysis is required for M1 macrophage polarization. Peritoneal macrophages (PEMs) sorted from C57BL/6 mice were stimulated with LPS (100 ng/mL) for 24 h, with or without glycolysis inhibitor, 2-DG (1 mM). The respiratory burst was determined by measuring the PPR and the protein expression of Glut1 (A,B). Pro-inflammatory cytokines TNFα and IL-12p40 were determined by flow cytometry assays (C–D). Cells from bone marrow (BMs) were induced with L929 supernatant for 7 days. After stimulation with LPS for 24 h, the expressions of pro-inflammatory cytokines TNFα and IL-12p40 and co-stimulator molecules CD80 and CD86 were determined with flow cytometry (E–H). iNOS expressions were analyzed with Immunoblotting and flow cytometry (I,J). Representative results are based on one of three independent experiments performed with similar results. The data are presented as the mean ± SD (n = 3–5 mice per group). Statistical significance was measured by one-way ANOVA for comparisons among multiple groups. ***P < 0.001, compared with the indicated groups.