FIGURE 4.

Effects of pathogenic mutations on intracellular α-syn levels and cytotoxicity of extracellular PFF treatment in primary cortical neurons. Neurons were treated with 1 μM sonicated WT or pathogenic mutant PFFs at 37°C for 24 h. (A,B) Immunoblots and analysis showing levels of intracellular α-syn. (C,D) Percentages of cell death measured by LDH release after 2 and 4 days treatment with sonicated PFFs. (E,F) ROS assays after PFF treatment. Mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA followed by Dunnett’s test compared to WT; n = 3 independent experiments.