FIGURE 5.

Effects of pathogenic mutations on the intracellular α-syn levels and cytotoxicity of extracellular PFF treatment in primary microglia. Microglia were treated with 1 μM sonicated WT or pathogenic mutant PFFs at 37°C for 2 h (A,C,E,G) and 24 h (B,D,F,H). (A–D) Immunoblots and analysis of intracellular α-syn. (E,F) Cell viability assessed by LDH release. (G,H) Oxidative stress assessed by ROS assays. Mean ± SEM; *P < 0.05, **P < 0.01, one-way ANOVA followed by Dunnett’s test compared to WT; n = 3 independent experiments.