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. 2020 Jun 12;11:727. doi: 10.3389/fpls.2020.00727

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Copyright © 2020 Petrie, Zhou, Leonforte, McAllister, Shrestha, Kennedy, Belide, Buzza, Gororo, Gao, Lester, Mansour, Mulder, Liu, Tian, Silva, Cogan, Nichols, Green, de Feyter, Devine and Singh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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T-DNA regions of binary vectors tested in this study shown in the right border (RB) to left border (LB) orientation: (A) GA7; (B) GA7_mod-B; (C) GA7_mod-D; (D) GA7_mod-E; (E) GA7_mod-F; (F) GA7_mod-G; (G) pJP3129. The boxed region of the GA7 series is common to all constructs. Genes are: Micpu-Δ6D, Micromonas pusilla Δ6-desaturase; Pyrco-Δ6E, Pyramimonas cordata Δ6-elongase; Pavsa-Δ5D, Pavlova salina Δ5-desaturase; Picpa-ω3D, Pichia pastoris ω3-desaturase; Pavsa-Δ4D, P. salina Δ4-desaturase; Lackl-Δ12D, Lachancea kluyveri Δ12-desaturase; Pyrco-Δ5E, P. cordata Δ5-elongase; PAT, phosphinothricin N-acetyltransferase from Streptomyces viridochromogenes. Promoters are: FP1, truncated napin promoter from Brassica napus; FAE1, Arabidopsis thaliana FATTY ACID ELONGATION1 promoter; Cnl1, Linum usitatissimum CONLININ1 promoter; Cnl2, L. usitatissimum CONLIIN2 promoter; 35S, Cauliflower mosaic virus 35S promoter with duplicated enhancer region. Terminators are: NOS, Agrobacterium tumefaciens NOS; Lectin, Glycine max Lectin; Cnl1, L. usitatissimum conlinin1; Cnl2, L. usitatissimum conlinin2. MAR denotes the Rb7 matrix attachment region from Nicotiana tabacum.