Figure 10.

Macrophage polarization and lipid mediator pathway induction in mouse lungs exposed to MWCNTs or C60F. Mice were treated as described in Figure 2 legend and the lungs were examined. Macrophage polarization and lipid mediator pathway induction were examined by double immunofluorescence staining with anti-F4/80 (green) and anti-ALOX5AP (red) antibodies (A), or anti-F4/80 (green) and anti-ALOX15 (red) antibodies (B). Numbers of representative double positive cells per 100 cells were counted from captured images and shown as mean ± SEM (n = 3). Scale bar = 20 μm. *p < 0.05; **p < 0.01; ***p < 0.001.