Figure 8.

Quantification of type 1 and type 2 cytokines in lung tissue. Mice were treated as described in Figure 2 legend and the lung tissue extract was prepared. Twenty-five microliter of tissue extracts were used for multiplex immunoassays to determine the levels of type I cytokines IFN-γ, IL-1β, IL-6, and TNF-α (A), or type II cytokines IL-4, IL-13, and IL-10 (B). The concentration of each cytokine was presented as pg/mg protein after normalization with the amount of protein used and shown as mean ± SEM (n = 3). *p < 0.05, **p < 0.01.