Figure 11.

Nec-1 inhibits the levels of necroptosis-related proteins and PI-positive cells in astrocytes. Astrocytes were exposed to oxygen and glucose deprivation (OGD) for 6 h followed by reoxygenation for 24 h. (A) Astrocytes were treated with Nec-1 (100 μM) during OGD and OGD/Re. Cell death was assessed with propidium iodide (PI) and Hoechst staining (PI: red; Hoechst: blue). (B–D) Representative images from Western blotting (WB) analysis of the levels of RIP1K, p-RIP1K, RIP3K, p-RIP3K, MLKL, and p-MLKL in astrocytes. β-Actin protein was used as a loading control. Mean ± SD, n = 3. ^**P < 0.01, ^*P < 0.05 vs. non-OGD-Re24 h group; ^##P < 0.01 vs. OGD6 h-Re24 h group.