Figure 14.

SB216763 reduces the levels of necroptosis-related proteins in astrocytes. Astrocytes were exposed to OGD for 6 h and reoxygenation for 24 h. Astrocytes were treated with or without SB216763 (5 μM) during OGD and reoxygenation. (A–C) Representative images from Western blotting (WB) analysis of the levels of RIP1K, p-RIP1K, RIP3K, p-RIP3K, MLKL, and p-MLKL. Columns present data from the quantitative analysis of RIP1K, p-RIP1K, RIP3K, p-RIP3K, MLKL, and p-MLKL immunoblots, respectively. β-Actin protein was used as a loading control. Mean ± SD, n = 3. ^**P < 0.01, ^*P < 0.05 vs. non-OGD-Re24 h group; ^##P < 0.01, ^#P < 0.05 vs. OGD6 h-Re24 h group.