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. 2020 Jun 18;40(6):BSR20194389. doi: 10.1042/BSR20194389

Figure 4. AMPKα is targeted by miR-301a-3p.

Figure 4

(A) AMPKα is predicated to be a target of miR-301a-3p using miRDB database. (B) Binding sites between AMPKα and miR-301a-3p were analyzed using TargetScan database. (C) Relative luciferase activity of the AMPKα-WT and AMPKα-MUT reporter plasmids in NCI-H1650 cells after transfection with miR-301a-3p mimics. (D) Relative protein expression of p-AMPKα in NCI-H1650 cells after transfection. AMPKα is revealed to be a target of miR-301a-3p and miR-301a-3p overexpression inhibits the phosphorylation level of AMPKα. Data represent the mean ± SD (n=3). *P<0.05 vs control, #P<0.05 vs mimic-NC, &P<0.05 vs inhibitor-NC.