(A) Relative expression of miR-301a-3p was detected using qRT-PCR, (B) relative mRNA and protein expression of AMPKα1 were detected using qRT-PCR and Western blot, respectively, (C) glucose level was detected using the corresponding kit, and (D) lactic acid level was detected using the corresponding kit in NCI-H1650 cells. AMPK agonist inhibited the expression of miR-301a-3p, glucose level, and lactic acid level after they were promoted by miR-301a-3p mimic. Data represent the mean ± SD (n=3). #P<0.05 vs miR-mimic + AICAR, &P<0.05 vs miR-inhibitor + BML-275.