Skip to main content
. 2020 Jun 18;40(6):BSR20200210. doi: 10.1042/BSR20200210

Figure 3. The expression of DDIT4 transcripts in human DPSCs exposed to TEGDMA on ECM substrate.

Figure 3

Schematic of the smRNA FISH technique (A). Expression of GAPDH (B,C) and DDIT4 (D,E) transcripts (white dots) in non-exposed hpDPSCs (B,D,F) and hpDPSCs exposed to 1.5 mmol/l TEGDMA (C,E,G). Phase contrast images of the non-treated (F) and treated (G) cells (arrows point to small vacuoles). The mRNA molecules of GAPDH (H) and DDIT4 (I) in treated and non-treated cells (**P≤0.01, ***P≤ 0.001). Comparison of the cell sizes of both samples (J). Fluorescent images of treated (K) and non-treated (L) hiDPSCs expressing DDIT4 transcripts with the corresponding phase-contrast images inserted. Bar scale = 10 μm.