Skip to main content
. 2020 Jun 12;11:1293. doi: 10.3389/fmicb.2020.01293

FIGURE 1.

FIGURE 1

Viral mRNA expression in A549 cells and mouse lungs and pathological changes of mouse lungs at indicated time after H1N1pdm09 infection. (A–D). Viral HA (A), NP (B), M1 (C), and NS1 (D) mRNA copies in A549 cells at 12, 24, 36, 48, and 72 h after infection with 1 × 103 TCID50 H1N1pam09 (n = 9). (E–H) Viral HA (E), NP (F), M1 (G), and NS1 (H) mRNA copies in mouse lungs at 3, 5, 7, and 10 days after infection with 1.5 × 104 TCID50H1N1pam09 (n = 5; data of 3 and 5 d.p.i.: refer to left Y axis; data of 7 and 10 d.p.i.: refer to right Y axis). (I) H&E-stained lung sections collected at 0, 3, 5, 7, and 10 days after infection (original magnification: ×100, scale bar = 200 μm, n = 5). Representative data shown from three independent experiments. P-values are indicated in the panels. HA, hemagglutinin; NP, nucleoprotein; M1, matrix protein 1; NS1, non-structural 1 protein.