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. 2020 Jun 12;11:1293. doi: 10.3389/fmicb.2020.01293

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Copyright © 2020 Bao, Wang, Liu, Zou, Zheng, Yu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Apoptosis analysis in the lungs of H1N1pdm09-infected mice by Gal-1 treatment. (A) Staining of lung sections with TUNEL to detect the apoptotic cells in the lungs collected at 6 d.p.i. (n = 3, original magnification, ×200, scale bar = 100 μm; green, TUNEL; blue, DAPI counterstaining of cell nuclei.). (B) TUNEL-positive cells in mouse lungs were quantified by averaging the number of positive stained cells in six randomly selected fields in each section (n = 3). (C) Apoptotic nuclear morphology observed by transmission electron microscopy (n = 4, original magnification, left panel: ×1750, scale bar = 5 μm; right panel: ×5900, scale bar = 1 μm; yellow arrow head: chromatin-condensed cells; red arrow head: condensed chromatin). Representative data shown from at least two independent experiments. P-values are indicated in the panels. DAPI, 2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride.