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. 2020 Jun 12;11:1293. doi: 10.3389/fmicb.2020.01293

FIGURE 6.

FIGURE 6

Viral mRNA expression, viral titers and immunohistochemical examination of mice lungs in different groups. (A–D) The fold change in viral HA (A), NP (B), M1 (C), and NS1 (D) mRNA levels in mouse lungs treated with Gal-1 compared with untreated controls (n = 6). (E) Viral titers in Gal-1-treated mouse lung homogenates compared with untreated controls (n = 8). (F) Immunohistochemical examination of NP-positive cells in mouse lung sections from different groups at 6 d.p.i. by rabbit polyclonal antibody for influenza A virus NP (original magnification, top panel: ×100, scale bar = 200 μm, middle panel: ×200, scale bar = 100 μm, bottom panel: ×400, scale bar = 50 μm; red arrow head: NP-positive cells). (G) NP-positive cells in mouse lungs were quantified by averaging the number of positive stained cells in six randomly selected fields in each section (n = 5–6). Representative data shown from at least two independent experiments. P-values are indicated in the panels.