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. 2020 Jun 8;36:101604. doi: 10.1016/j.redox.2020.101604

Fig. 4.

Fig. 4

Antioxidant rescues proliferation ability and reduces oxidative DNA damage of RAD51 knockdown cells. (A) Clonogenic assay of A2780 cells transfected siScr or siRAD51 treated with or without 10 mM NAC. (B) Cell viability was measured by CCK-8 assay. A2780 cells transfected siScr or siRAD51 treated with or without 10 mM NAC. (C) EdU incorporation of A2780 cells transfected siScr or siRAD51 treated with or without 10 mM NAC. (D) Clonogenic assay of A2780 cells treated with or without 5 μM B02 and 10 mM NAC. (E) Western blot analysis of γ-H2AX protein levels in A2780 and HEY cells transfected siScr or siRAD51 treated with or without 10 mM NAC. (F) Western blot analysis of γ-H2AX protein levels in HO8910 cells stably transfected RAD51 shRNA treated with or without 10 mM NAC. α-Tubulin was used as a loading control. Band intensities were quantified by ImageJ. Data presented as mean ± S.D. are representative of three independent experiments. The statistical differences between the two groups were analyzed by two-sided unpaired Student's t-test (**p < 0.01, ***p < 0.001, ns stand for no significant).