Figure 6.

Hypoxanthine Protects MSP-Depleted Mice Against DSS Colitis by Supporting Energy Balance and Proliferation

(A) Weight curves of streptomycin-treated, conventionally raised control (S-0 mM HPX-DSS) and 1 mM hypoxanthine-supplemented (S-1 mM HPX-DSS) mice subjected to DSS. Data are represented as mean ± SEM (n = 5).

(B) Colon lengths of the hypoxanthine-supplemented, DSS-treated mouse cohort. Data are represented as mean ± SEM (n = 5).

(C) Colon tissue adenylate energy charges of the hypoxanthine-supplemented, DSS-treated mouse cohort. Data are represented as mean ± SEM (n = 5).

(D) Representative hematoxylin and eosin (H&E) staining images of the hypoxanthine-supplemented mouse cohort. Yellow arrows indicate goblet cell theca, red bars represent crypt depth, and the black arrow highlights the lack of epithelial cell density.

(E) Mucosal depth measurements of the hypoxanthine DSS mouse cohort. Data are represented as mean ± SEM (n = 44).

(F) Immunofluorescent analyses of S-0 mM HPX-DSS and S-1 mM HPX-DSS mice targeting Ki67. Data are represented as mean (n = 21).

(G) Representative Ki67 immunofluorescent images of the hypoxanthine-supplemented, DSS-treated mouse cohort.

(H) Distribution of mucosal TUNEL-positive mucosal cells across the hypoxanthine DSS mouse groups. Data are represented as mean ± SEM (n = 4).

∗p < 0.05, ∗∗∗p < 0.001. Scale bars, 50 μm.