Figure 4.

Comparison of SVF proliferation, spheroid volume and formation, and adipogenic functionality in 2D culture and ObaGel. (a) Bright field and confocal microscopy images of SVF cell proliferation in 2D culture and ObaGel. Cells were seeded in traditional 2D media or ObaGel, cultured for 5 days, and stained with the Hoechst dye. Robust cell proliferation occurs in ObaGel compared to 2D culture, as indicated by Hoechst positivity. (b, c) report photomicrographs and respective quantification that reflect a positive correlation between ObaGel concentration and SVF spheroid size, density, and volume within 5-7 days of culture. (d) demonstrates that adipogenesis occurs within spheroids as early as day 3 of differentiation. Values reported as mean ± SD. ^∗p < 0.05 when compared to the freshly thawed cells or silk constructs.