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. 2020 Jun 12;11:556. doi: 10.3389/fphys.2020.00556

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Copyright © 2020 Nielsen, Frandsen, Jensen, Prokhorova, Dalgaard, Bech, Nygaard, Suetta and Aagaard.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative skeletal muscle cross sections displaying immunoreactivity for NG2 (red), CD31 (green), and 4’,6-diamidino-2-phenylindole (DAPI) (nuclear stain) (1A,1B) and Laminin (2A–2C). 1A,1B: Samples are from blood flow restriction exercise (BFRE) at baseline (Pre, 1A) and 8 days into the intervention (Mid8, 1B). Note the increase in the area of CD31C structures and myofibers as well as a similar number of CD31C structures, suggesting a stable capillary density (CD) and an increase in capillary/fiber and capillary cross-sectional area. 2A: Normal laminin morphology, baseline (Pre); 2B/2C: lowly/highly increased perivascular laminin immunoreactivity relative to baseline (2A).