Table 1.
List of primers and synthetic genes used for pvcrt and pvmdr1 gene copy number determination and sequencing
| Name | Sequence (5′→3′) | Reference |
|---|---|---|
| Gene copy number determination | ||
| pvmdr1 synthetic gene | GCAACTCCATAAAGAACAACATCAAGTATAGTTTGTACAGCCTGAAAGATTTAGAAGCCTTATCGGAGGAGTCGAACGAAGATGGTTTTTCTTCTCAAA | this work |
| β-TubulinPv synthetic gene | CAGGAGTTACATGTTCGTTAAGATTTCCTGGTCAGTTAAATTCTGATTTGAGAAAATTAGCTGTCAATTTAATTCCCTTCCCAAGACTCCACTTTTTTATGATTGGTTTTGCACCACTAACAAGCAGAGG | 20 |
| PvMDR1_F | GCAACTCCATAAAGAACAACATC | this work |
| PvMDR1_R | TTTGAGAAGAAAAACCATCTTCG | this work |
| PvCRT_F | GGGAGTCCCCAAATAACCCC | this work |
| PvCRT_R | GTTGTCTCGCCACTCTCCTG | this work |
| CN_β-tubulin_F | CATGTTCGTTAAGATTTCCTGGT | 20 |
| CN_β-tubulin_R | GTTAGTGGTGCAAAACCAATCA | |
| Sequence polymorphism | ||
| Pvmdr976 F | GGATAGTCATGCCCCAGGATTG | 3 |
| Pvmdr976 R | CATCAACTTCCCGGCGTAGC | |
| Pvcrt-o F1 (sense) | AAGAGCCGTCTAGCCATCC | 11 |
| Pvcrt-o R3 | AGTTTCCCTCTACACCCG | |