Figure 2.

Schematic representation of the optrA gene loci in E. faecalis and E. faecium clinical isolates from Ireland. Different variants of the genetic environment surrounding optrA were detected in LRE encoded on plasmids (a) and within the chromosome (b) and were aligned against the prototype optrA_WT, first described by Wang et al. in 2015.⁹ Genes of interest and their orientation are shown with arrows and labelled; red indicates antibiotic resistance genes, green indicates ISs/transposases, blue indicates known proteins and grey indicates hypothetical proteins. Tnp, transposase; met, truncated DNA adenine methylase; uvrX, putative UV-damage repair protein; D, DNA-directed RNA polymerase β subunit; Ferr, ferredoxin; N, NAD(P)H oxidoreductase.